Effect of hydrogen ion-gradient on carrier-mediated transport of glycylglycine across brush border membrane vesicles from rabbit small intestine.

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Effect of hydrogen ion-gradient on carrier-mediated transport of glycylglycine across brush border membrane vesicles from rabbit small intestine.

Glycylglycine (Gly-Gly) transport across rabbit small intestinal brush border membrane vesicles was studied in the presence and absence of a Na+ gradient or a pH gradient. The transport was found to be entirely independent of Na+ but significantly stimulated by lowering extravesicular pH (pHo). The maximum stimulation was seen at pHo 5.5, where the uptake rate was about 2-times higher than the ...

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In previous studies, sulfoxide metabolite was observed in animal and human intestinal perfusions of cimetidine and other H2-antagonists. A sequence of follow-up studies is ongoing to assess the intestinal contributions of drug metabolism and drug and metabolite transport to variable drug absorption. An evaluation of these contributions to absorption variability is carried out in isolated fracti...

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Amino acid transport in brush-border-membrane vesicles isolated from human small intestine.

Uptake of L-alanine and L-phenylalanine by purified bursh-border-membrane vesicles isolated from human small intestine was investigated by using a rapid-filtration technique. L-Alanine entered the same osmotically reactive space as D-glucose, indicating that transport into the vesicle rather than binding to the membranes was being observed. The uptake rate for L-alanine was higher in the presen...

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Carrier-mediated L-lactate transport in brush-border membrane vesicles from rat placenta during late gestation.

The mechanism for L-lactate transport across microvillous membrane vesicles prepared from rat placenta was examined. Uptake of L-lactate into these vesicles was mainly the result of transport into the intravesicular (osmotically active) space. The initial rate of L-lactate uptake was not affected by the presence of an inward gradient of either Na+ or K+. In the presence of an inward-directed pr...

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Simultaneous isolation and characterization of brush border and basolateral membrane vesicles from bovine small intestine.

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ژورنال

عنوان ژورنال: The Japanese Journal of Physiology

سال: 1985

ISSN: 1881-1396,0021-521X

DOI: 10.2170/jjphysiol.35.629